Abstract
ABSTRACT
Cholesterol oxidase is an alcohol oxidoreductase flavoprotein with wide biotechnological applications. In recent years, microbial cholesterol oxidases have gained great attention due to its widespread applications in many industries.This research is aimed to produce extracellular cholesterol oxidase as well as its purification. Soil sample was collected from Mungadi fadama land area and Streptomyces was isolated using starch casein aga media. The isolates (Streptomyces) was identified using their physical and microbial characteristics and screened for the potentiality of producing cholesterol oxidase using red coloring stain method, then inoculated into production media for the production of cholesterol oxidase. After production period, the protein content of the culture medium was determined as well as the enzyme activity using modified Yazid method. The enzyme was partially purified using ammonium sulphate method. The morphological and microbial characteristics (Gram positive, earthy ordour, white in colour, rod shaped and no motility) showed by the selected isolates, clearly indicate that, the isolated bacteria was Streptomyces as described in standard Streptomyces project. The activities of enzyme was 0.92 U/mL with control 0.11 u/ml. The specific activity was 83U/mg with yield 29.64% after one step ammonium sulphate precipitation. Bacterial Streptomyces from Mungadi Fadama land soil was capable of producing cholesterol oxidase enzyme and the purified enzyme can be used for many industrial applications.

Key words: Key wards: Streptomyces, Cholesterol Oxidase, Production,Purification